Quantitative Spectrophotometric Microplate Assay for Angiotensin-converting Enzyme in Cerebrospinal Fluid

Quantitative spectrophotometric microplate assay for angiotensin-converting enzyme in cerebrospinal fluid.

will address this hypothesis, regarding which little information is currently available. Similarly, we have no useful information at present regarding possible interactions between S100B and sex hormones, which could also be hypothesized on the basis of the gender differences found in S100B concentrations. More generally, the growing body of evidence that indicates a biological role of S100B as...

Direct spectrophotometric assay for angiotensin-converting enzyme in serum.

The appropriate conditions for determining angiotensin-converting enzyme in human serum with use of 2-furanacryloyl-L-phenylalanylglycylglycine as substrate are described. The method, a modification of that reported by Holmquist et al. (Anal. Biochem. 95:540-548, 1979) for purified rabbit lung enzyme, is based on the blue shift of the absorption spectrum that occurs upon hydrolysis of the subst...

Characterization of a spectrophotometric assay for angiotensin converting enzyme.

Although serum angiotensin converting enzyme (EC 3.4.15.1) activity has generally been shown to be increased in patients with sarcoidosis, considerable variation in the diagnostic usefulness of this test has been reported. We investigated the possibility that this variation may be the result of inhibition of the widely used spectrophotometric assay by various substances. We also prospectively e...

Angiotensin Converting Enzyme in Sarcoidosis

Inhibitor binding assay for angiotensin-converting enzyme.

We describe a new principle for the determination of enzymes, here applied to angiotensin-converting enzyme (ACE, EC 3.4.15.1) in human serum. The enzyme inhibitor binding assay is based on specific binding of labeled inhibitor to the active center of the enzyme. Serum (10-15 microL) is incubated with 125I-labeled ACE inhibitor (" 351A ," a p- hydroxybenzamidine derivative of N-(1-carboxy-3-phe...